fixable viability dye efluor780 (Thermo Fisher)

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Thermo Fisher fixable viability dye efluor780
Fixable Viability Dye Efluor780, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fixable+viability+dye+efluor780/PBS/pm41273303-207-9-13
Average 99 stars, based on 1 article reviews

fixable viability dye efluor780 - by Bioz Stars, 2026-09

99/100 stars

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Staining:

Article Title: What Is the Role of Giant Endosomal Sorting Complexes Required for Transport (ESCRT) Structures in T Cell Activation?
Article Snippet: .. The next day all cell samples were stained with Fixable Viability Dye eFluor780 (eBioscience, 1:2000 in cold PBS, 100 μL) on ice for 20 min followed by washing with PBS and staining for several surface expression markers MHC-I, MHC-II and CD86 by incubating 105 cells in 30 μL/well with corresponding antibodies (Table 2) for 30 min at 4◦C in the dark. .. Cells were washed three times with PBS prior to acquisition, and they finallywere resuspended in 100 μL/well PBS and analyzed with flow cytometry (CytoFlex S flow cytometer, Beckman Coulter Inc., Indianapolis, IN, USA).

Article Title: Effectorless Fc-fusion improves FLT3L drug-like properties for cancer immunotherapy combinations.
Article Snippet: Cells were then harvested and stained with Human TruStain FcX (Biolegend) followed by anti-CD11c BV421 (clone Bly6, BD Biosciences) and anti-CD141 PE (clone AD514H12, Miltenyi Biotec). .. Cells were washed and stained with Fixable Viability Dye eFluor780 (Thermo Fisher Scientific) and fixed in PBS + 1% PFA. .. Samples were acquired on an Attune NxT flow cytometer (Thermo Fisher Scientific) and data analysed with FlowJo (BD Biosciences).

Article Title: What Is the Role of Giant Endosomal Sorting Complexes Required for Transport (ESCRT) Structures in T Cell Activation?
Article Snippet: .. Before the fixation step, all cell samples were stained with Fixable Viability Dye eFluor780 (eBioscience, 1:2000 in cold PBS, 100 μL) on ice for 10 min followed by 100 μL/per well wash with PBS containing 1% BSA. .. The cells were stained for the CD3 receptor with 25 μL mouse anti-CD3 APC-conjugated antibody (eBioscience, Catalog # 17-0038-42, 1:100 in 1% BSA) on ice for 30 min. After washing with 100 μL PBS the cells were resuspended in 100 μL/well PBS and analyzed with flow cytometry.

Article Title: Inflammation Promotes Proteolytic Processing of the Prohormone Chromogranin A by Macrophages
Article Snippet: For the internal cyclooxygenase-2 (COX-2) staining and metabolic probe treatment, 2 × 10 6 isolated human CD14 + monocytes were cultured in ultra-low adherent 6-well plates (Corning, CLS3471-EA) for 7 days containing 2 mL of complete media (RPMI 1640, 10% FBS, 2 mM L-glutamine, 1% Antibiotic-Antimycotic) containing 100 ng/mL recombinant human M-CSF protein and 200 nM human PST or 1 μM human CST at 37 °C and 5% CO 2 . .. For the internal COX-2 staining, 100 000 cells per well were seeded in a 96-V-bottom plate and stained with Fixable Viability Dye eFluor780 (eBioscience, 65-0865-14, 1:1000 in cold PBS) for 15 minutes on ice prior to fixation with 4% PFA for 15 minutes at 4 °C. ..

Expressing:

Immunopeptidomics:

other:

Article Title:
Article Snippet: 3 nature portfolio | rep orting sum m ary M arch 2021 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Antigen conjugate, clone, distributor, catalogue number, LOT number In vivo antibodies mCD4, GK1.5, BioXCell, BE0003-1, LOT: 699918O1B mCD8a, 2.43, BioXCell, BE0061, LOT: 624616A2 mNK1.1, PK136, BioXCell, BE0036, LOT: 735620O1 Immunohistochemistry antibodies mCD8, PE, 56-6.7, Invitrogen, 12-0081-82, LOT: 2207581 Flow cytometry antibodies mCD3 BV711, 17A2, BioLegend, 1100241, LOT: B245637 mCD45 BV510, 30-F11, BioLegend, 103138, LOT: B333193 mCD11b PE-dazzle, M1/70, BioLegend, LOT: B316038 mCD3 BV711, 17A2, BioLegend, 1100241, LOT: B245637 mCD4 PE Texas Red, RM4-5, Invitrogen, MCD0417, LOT: 1837385 mCD8 AF700, 53-6.7, BioLegend, 100730, LOT: B326021 mF4/80, BV421, BM8, BioLegend, 123137, LOT: B295586 mMHCII AF700, M5/114.15.2, Biolegend, 107606, LOT: B264454 mPD-L1, BV605, 10F.9G2, BioLegend, 124321, LOT: B245034 mLy6C, FITC, HK1.4, BioLegend, 128006, LOT: B217035 mLy6C, APC, HK1.4, BioLegend, 128016, LOT: B274271 mFoxP3, FITC, FJK-16s, Invitrogen, 11-5773, LOT: 2007700 mLag3, PE-Cy7, eBioC9B7W, Invitrogen, 25-2231-82, LOT: 4284448 mCD45, APC-Cy7, 30-F11, BD Biosciences, 557659, LOT: 9003933 mCD25, PerCPCy5.5, PC61, BioLegend, 102030, LOT: B198863 mPD-L1, BV711, 10F.9G2, BioLegend, 124319, LOT: B309517 mPD-1, BV421, 29F.1.A12, BioLegend, 135218, LOT: B256183 mKi67, eFlour450, SolA15, Invitrogen, 48-5698-82, LOT: 1998365 mNK1.1, Vio Bright B515, REA1162, Miltenyi Biotec, 130-120-503, LOT: 5900109216 CD38, FITC, 90, BioLegend, 102705, LOT: B235499 CD86, PE, GL-1, BioLegend, 105007, LOT: B248265 CD64, PerCP-eFluor 710, X54-5/7.1, Invitrogen, 46-0641-80, LOT: 2161982 MHC II, PE-Cy7, M5/114.15.2, BioLegend, 25-5321-82, LOT: 4332615 MerTK, Brilliant Violet 421, 108928, BD Biosciences, 747837, LOT: 8333892 CD172, APC/Cy7, P84, BioLegend, 144017, LOT: B258707 CD71, Brilliant Violet 510, RI7217, BioLegend, 113823, LOT: B305768 fixable viability dye eFluor780, eBioscience, 65-0865, LOT: 1977883 fixable viability dye eFluor520, eBioscience, 65-0867, LOT: 1915314 CD16/CD32 Monoclonal Antibody, unconjugated,, eBioscienceProduct 11-4811-85 Validation data of flow cytometry and immunohistochemistry antibodies can be found on the suppliers' website, e.g. in technical data sheets.

Incubation:

Article Title: A Singular Base Editing Platform for Polyfunctional Multiplex Engineering of Immune Cells
Article Snippet: The cells were incubated at 37 °C with 5% CO2 in the presence of a CD107a antibody for 1 hr followed by a treatment with brefeldin A (10 μg/mL; BD Biosciences) and monensin (0.7 μg/mL; BD Biosciences). .. After 6 hrs of incubation at 37 °C with 5% CO2, the cells were collected, and incubated with Fixable Viability Dye eFluor780 (eBioscience) and human TruStain FcX (BioLegend) for 10 mins at RT. ..

Article Title: Different CMV-specific effector T cell subtypes are associated with age, CMV serostatus, and increased systolic blood pressure.
Article Snippet: 1 × 106 cells were incubated with MHCI-tetramers, consisting of tetramerized and PE-labelled HLA-A*02:01 monomers carrying the NLVPMVATV peptide CMVpp65495 − 503 (1002-07 ImmunAware, Horsholm, Denmark; IEDB#: 44920) at room temperature for 30 min. .. Subsequently, anti-CD8-BV510 (clone SK1, Biolegend 344732), anti-CD27-APC (clone O323, Biolegend 302810), anti-CD45RO-PacificBlue (clone UCHL1, Biolegend 304216) and a viability dye (ThermoFisher/Invitrogen/eBioscience fixable viability dye eFluor780) were added and incubated for 30 min at 4 °C. ..

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